Fig. 8

MNQ rescues tetracyclines activity in vivo. Scheme of the experimental protocols for the thigh infection model induced by E. coli 47EC (a) and E. coli DH5α + pAM401-tet(X4)-evoked systemic infection model (d). b In the mouse thigh infection model, bacterial load in infected thigh muscle were markedly decreased by combination therapy (n = 8 per group). c Histopathology and macroscopic observation of the infected thigh muscle. e Additionally, in the systemic infection model, the bacterial burden in the livers and spleens of mice challenged with a intraperitoneal inoculation of E. coli DH5α + pAM401-tet(X4) (n = 10 per group). f H&E staining for the histopathological changes of mice heart, liver, spleen, lung and kidney at 48 h post-infection. And the typical pathological lesions were marked using white arrow