Fig. 1
From: An automated fluorescent immunoassay for on-site screening of AFM1 in raw milk at the ppt level

Optimization of the physicochemical parameters on the ic-ELISA and the calibration curves assessed by the ic-ELISA. The effect of (A) ionic strength, (B) pH value, (C) methanol on the ic-ELISA. (D) acetonitrile on the ic-ELISA. (E) The calibration curve of ic-ELISA for AFM1 based on mAb 10F8. (F) Cross-reaction of AFM1 with various mycotoxin candidates based on mAb 10F8