Fig. 2

Identification of candidate proteins labeled by TurboID (A). The volcano map showing the identified proteins by proximal biotin labeling strategy. The red dots represented significantly up-regultaed proteins by comparison with the parental RH line. Five of the candidate proteins for subsequent analysis were labelled in the plot (See Table S1). B The candidate proteins were analyzed based on ToxoDB and InterPro databases. C Localization of SFA4-6HA (green) and SFA5-6HA (green) in the background parasite line SFA2-6Ty. The HA fusion was stained green while the SFA2Ty was stained red as a reference. The Pearson Correlation coefficient (PCC) was analyzed with mean ± SEM using the intensities over the white line using the NIS software. Three independent experiments were performed with similar outcomes, and The PCC shown were averages and standard deviation (N = 20). Scale bar = 2 μm. D The model pattern of SFA2 and two novel SFA proteins in T. gondii. The segmented Coiled Coil Domain are conserved and drawn as yellow boxes. E Robust Multi-array Average (RMA) values of transcripts endocoding SFA2 and two novel proteins over two consecutive division cycles based on previous published data from Behnke and co-workers [19]. F Parasites expressing SFA2-6Ty and SFA5-6HA were scored by IFA using anti-Ty and anti-HA antibodies in an asynchronous parasite population (n = 200) for parasites grown in HFF cells for 24 h. The data of image were calculated based on two independent experiments. Error bars represent the standard error