Fig. 3

Porcine ASC-derived intestinal organoids. A Representative images of the time course of porcine enteroid differentiation from intestinal crypts. During culture in Matrigel, small spheroids form on day 3 after crypt isolation, gradually mature over time, and form budding-like crypt structures on day 7 [22]. BÂ Representative images of the time course of porcine colonoid development. Colonic crypts were isolated and differentiated into budding-like colonoid structures on day 15 [22]