Fig. 1

Schematic illustration and construction of the recombinant SEE protein. A A schematic map of SEE-Pet28a. B SDS-PAGE analysis of the purification of recombinant SEE protein. Lane 1: Binding buffer. Lane 2–5: SEE proteins eluted by 30 mM, 80 mM, 120 mM and 500 mM imidazole, respectively. M: molecular weight marker