Fig. 1

Characterization of Phage P5054. A Phage P5054 forms clear plaques surrounded by translucent halos on the double-layer agar plate, and the halos surrounding the plaques increase in size after 5 days. B Spot tests of Klebsiella pneumoniae strain 8665 and its uncapsulated mutant strain 8665Δwbap by phage P5054 and depolymerase. Phage P5054 and depolymerase K57-Dpo8 (0.75 mg/mL) were spotted on the bacterial lawns. K64-ORF41 (0.75 mg/mL), a depolymerase that targeted K64-type capsular polysaccharide [19], was used as a negative control