Fig. 5

Antibiofilm activity of K57-Dpo8 against K. pneumoniae strain 8665. A Inhibition of biofilm formation by K57-Dpo8. K. pneumoniae strain 8665 was incubated with K57-Dpo8 or control depolymerase K64-ORF41 at 10 μg/mL in 96-well plates for 48 h. Residual biofilm biomass was assessed by crystal violet staining, with absorbance measured at 595 nm. B Disruption of mature biofilm by K57-Dpo8. Biofilms formed by K. pneumoniae strain 8665 in 96-well plates over 48 h were treated with K57-Dpo8 or control depolymerase K64-ORF41 at 10 μg/mL for an additional 3 h. Residual biofilm biomass was evaluated by crystal violet staining with absorbance measured at 595 nm. Data are presented as the mean ± SEM and represent three separate experiments. Two-tailed unpaired Student’s t-test was used for statistical analysis. * p < 0.05; ** p < 0.01