A novel depolymerase encoded by phage P5054 specifically degrades the K57-type capsular polysaccharide of Klebsiella pneumoniae

Table 1 Host spectrum of phage P5054 and depolymerase K57-Dpo8

Isolate No	Capsular genotype	ST	P5054		K57-Dpo8
			Plaques	Halos
8665¹*	K57	ST412	+	+	+
GX33²	K57	ST412	+	+	+
TJ55²	K57	ST412	+	+	+
JH17²	K57	ST218	+	+	+
TJ2²	K57	ST218	+	+	+
GX19²	K57	ST592	+	+	+
TJ11²	K57	ST592	+	+	+
19–249³	K47	ST11	-	-	-
18–81³	K47	ST11	-	-	-
16–312³	K64	ST11	-	-	-
20–445³	K64	ST11	-	-	-
6113¹	K1	ST23	-	-	-
20–256³	K19	ST15	-	-	-
18–746³	K19	ST15	-	-	-
20–300³	K25	ST11	-	-	-
19–618	K51	ST231	-	-	-

The K-types of all strains were determined with wzi sequencing. The sequence types (ST) were evaluated using the Institut Pasteur MLST database [1]. The lytic spectrums of phage P5054 and depolymerase K57-Dpo8 were assessed on all 16 K. pneumoniae isolates using drop tests (-, no plaques or halos; + , showed plaques or halos)
¹Strains were isolated from Huashan Hospital affiliated with Fudan University [18]
²Strains were isolated from the Second Affiliated Hospital Zhejiang University School of Medicine
³Strains were isolated from Ruijin Hospital affiliated with Shanghai Jiao Tong University School of Medicine [9]
^*Host strain of phage P5054

ISSN: 2731-9970