Table 2 List of primers
Name | Sequence (5’–3’) | Purpose |
|---|---|---|
K57-P5054-F | TTAAGAAGGAGATATACATATGTTAAACACTTTGAATCAACCGAA | Amplification of K57-Dpo8 |
K57-P5054-R | GTGGTGGTGGTGGTGGTGCTCGAGGTCCCCAAGGTAGACCAGT | Amplification of K57-Dpo8 |
K57-wbap-F1-pSUMO | CAGCAGCAGACGGGAGGATCCTATCTGATATTTTATCTGGCCAA | Construction of wbap mutant plasmids |
K57-wbap-R1-pSUMO | AGAGCGCTTTTGAAGCTAATTAGTTACACGCCTACTTATGAT | Construction of wbap mutant plasmids |
K57-wbap-F2-pSUMO | ATCATAAGTAGGCGTGTAACTAATTAGCTTCAAAAGCGCTCT | Construction of wbap mutant plasmids |
K57-wbap-R2-pSUMO | TTGTTTTTATCCAAGCATAATGAACGGTACCAATTGGGGATCTT | Construction of wbap mutant plasmids |
K57-wbap-F3-pSUMO | AAGATCCCCAATTGGTACCGTTCATTATGCTTGGATAAAAACAA | Construction of wbap mutant plasmids |
K57-wbap-R3-pSUMO | CTCGAGTGCGGCCGCAAGCTTACCACTAATTAGAACCGCCC | Construction of wbap mutant plasmids |
K57-wbap-F1 | TATCTGATATTTTATCTGGCCAA | Construction of wbap mutant plasmids |
K57-wbap-R1 | ACCACTAATTAGAACCGCCC | Construction of wbap mutant plasmids |
K57-wbap–check-F | GGAGCTAGTCCTAATGCGGG | Construction of wbap mutant plasmids |
K57-wbap-check-R | GCCAACTGAACAACGCTTA | Construction of wbap mutant plasmids |
wzi-F | GTGCCGCGAGCGCTTTCTATCTTGGTATTCC | Determination of capsular types |
wzi-R | GAGAGCCACTGGTTCCAGAAYTTSACCGC | Determination of capsular types |